Season ticket holders and West Ham members secured every seat at the London Stadium even before the club released general admission tickets.
The Hammers will face the French in the first leg of their first European quarter-final since 1981 on Thursday, April 7.
With the 2-0 round of 16 second-leg win over Sevilla having seen the greatest atmosphere the London stadium had produced since the move from West Ham, demand was at its peak for the Lyon fixture, with the club facing a level of interest and calls like never before.
Goals from Tomas Soucek and Andriy Yarmolenko saw West Ham overcome a 1-0 first-leg deficit against the six-time winners after extra time last week.
The Sevilla match saw the biggest crowd ever at West Ham for a home game with 60,000 in attendance, which is expected to be matched by Lyon’s draw.
Manager David Moyes said this week: “When I arrived I said the most important thing about West Ham was the potential and, I thought, untapped in many ways. Who would have said there is two years that we would have 60,000 here to get West Ham to the quarter-finals of the Europa League?And I see a big chance for things to improve even more.
“I think if you’re going to play us you know you’re in a game. We always want to challenge the best teams. Right now the place is blowing up. I see West Ham supporters everywhere I go and we look forward to another great night at the London Stadium.
Hammers fans who weren’t able to get a ticket could get another shot if Lyon return some of their allowance.
West Ham travel to Ligue 1 10th-placed Lyon for the second leg on Thursday April 14.